Single Protein Library: HSA

April 22, 2014

The Human Serum Albumin library was created from data generated at the NIST Mass Spectrometry Data Center.

Reference: J Proteome Res. 2013 Dec 6;12(12):5666-80. doi: 10.1021/pr400611h. Comprehensive analysis of protein digestion using six trypsins reveals the origin of trypsin as a significant source of variability in proteomics. Walmsley SJ1, Rudnick PA, Liang Y, Dong Q, Stein SE, Nesvizhskii AI. Consensus ion-trap fragmentation spectra for 3040 HSA peptides are available in two 'zipped' formats. Unzip after downloading.

File - Description Size Link - ZIP Archive for Installation of Windows Database Program and Library. The folder generated by extracting this package can be used directly by MS Search or MSPepSearch and by the MSPepSearch node within Proteome Discoverer. ~19MB Download - ZIP Archive with Library. Consensus library in plain ASCII text (msp format). This format can be used as an exchange format. It is also directly readable by some software applications, including Skyline, after unzipping. (.MSP text format) . ~6MB Download
md5_checklist.chk - MD5 hash file. 1KB Download

To install the Database Program and Library after downloading and unzipping, click on NIST14_Peptide_HSA_Library.exe and then follow instructions.

MS1 Statistics for HSA Peptide Identifications is available here.

Additional documentation for NIST peptide libraries is here.

For more installation details readme

© Copyright information
These peptide mass spectral libraries are protected by copyright law and may not be re-distributed without a valid Distribution Agreement. To receive such an agreement, contact the Standard Reference Data Program at the National Institute of Standards and Technology by emailing or calling 301-975-2008.