==== The NISTmAb Mass Spectral Library of Disulfide-Linked Peptides ====

As a result of a major upgrade of the earlier version of the spectral library, this 2021 New Release added 596 "best" individual spectra (Selected) of disulfide-bonded peptides, in addition to consensus spectra. The details can be found in {{:peptidew:lib:nist_disulfide_library_v21.1_release_note.pdf|the library new release note}} and {{:peptidew:lib:nist_disulfide_library_v21.1_readme.pdf|Readme}} PDF files.

The library is presented in two parts, including 321 and 907 spectra generated by Orbitrap Fusion Lumos (Lumos) and Q Exactive Hybrid Quadrupole-Orbitrap (QE) mass spectrometers, respectively. Altogether, there are 155 different peptides identified from native SS linkages in the NISTmAb and 207 different peptides detected from experiment-induced SS bonds.

<WRAP indent> <WRAP indent> **Reference**: Comprehensive Analysis of Tryptic Peptides Arising from Disulfide Linkages in NISTmAb and Their Use for Developing a Mass Spectral Library. J Proteome Res. 2021 Mar 5;20(3):1612-1629. Dong Q, Yan X, Liang Y, Markey SP, Sheetlin SL, Remoroza CA, Wallace WE, Stein SE. 
https://pubs.acs.org/doi/10.1021/acs.jproteome.0c00823

 </WRAP> </WRAP>


Two disulfide spectral libraries: \\ 
 
^  ^File ^Size ^Link to download |
| 1| The spectral library generated from Lumos|||
|  |**NISTmAb_Disulfide_Lumos_v211.zip** - Archive file with the Library in NIST MS Search format. Unzip whole folder where the NIST MS Search program installed.  If you do not have NIST MS Search Program see **//Notes//** below. |3.8MB |[[https://chemdata.nist.gov/download/peptide_library/libraries/disulfidepeptides/NISTmAb_Disulfide_Lumos_v211.zip|Download]] |
|  |**NISTmAb_Disulfide_LumosMSP_v211.zip** - Archive file with the Library in MSP (text) format. |1.2MB |[[https://chemdata.nist.gov/download/peptide_library/libraries/disulfidepeptides/NISTmAb_Disulfide_LumosMSP_v211.zip|Download]] |
| 2| The spectral library generated from Q-Exactive|||
|  |**NISTmAb_Disulfide_QE_v211.zip** - Archive file with the Library in NIST MS Search format. Unzip whole folder where the NIST MS Search program installed. If you do not have NIST MS Search Program see **//Notes//** below. |3.5MB |[[https://chemdata.nist.gov/download/peptide_library/libraries/disulfidepeptides/NISTmAb_Disulfide_QE_v211.zip|Download]] |
|  |**NISTmAb_Disulfide_QEMSP_v211.zip** - Archive file with the Library in MSP (text) format. |841KB |[[https://chemdata.nist.gov/download/peptide_library/libraries/disulfidepeptides/NISTmAb_Disulfide_QEMSP_v211.zip|Download]] |
|  |md5_checklist.chk - MD5 hash file. |1KB |[[https://chemdata.nist.gov/download/peptide_library/libraries/disulfidepeptides/md5_checklist.chk|Download]] |


** NOTES** \\
You will need **NIST MS Search software v.2.3**  to search the oligosaccharides library. The software can be download: {{:chemdata:nist17:nist17demo.zip|here (Download installation program)}}. Download archive and unzip installation file. Run NIST17Demo.exe to install the search program.


The Version v1 Release Date: September 19, 2020 \\
The Version v21.1 Release Date: March 8, 2021



<WRAP right lo>For questions, please contact  \\ Dr. Qian Dong  \\ (email: qian.dong@nist.gov) </WRAP>


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