==== MS Library of Oligosaccharides in NIST Human Milk SRM 1953 ====


The library contains


    * 470 positive and negative ion spectra of 74 oligosaccharides derived from human milk NIST Standard Reference Material 1953.
    * It includes lactose, 45 neutral oligosaccharides and 29 sialylated oligosaccharides.
    * All fragment ions of MS2 spectra are comprehensively annotated.
    * Fragmentation techniques: HCD and FT-IT


The development of MS library of annotated oligosaccharides from human milk reference material is discussed.


<WRAP indent> <WRAP indent> **Creating a Mass Spectral Reference Library for Oligosaccharides in Human Milk**     \\ Connie A. Remoroza, Tytus Mak, Maria Lorna A. De Leoz, Yuri A. Mirokhin, Stephen E. Stein   \\ //Mass Spectrometry Data Center, Biomolecular Measurement Division, National Institute of Standards and Technology, Gaithersburg, MD, 20899-8362, USA//    \\ 
[[https://pubs.acs.org/doi/10.1021/acs.analchem.8b01176|Analytical Chemistry, 2018  DOI: 10.1021/acs.analchem.8b01176]] </WRAP> </WRAP>


The MS library of oligosaccharides from SRM 1953 is available for download. 


^  ^File ^Size ^Link to download |
|1 |**MS_Library_of_Oligosaccharides_in_human_milk_SRM_1953.zip** - Archive file with the Library in NIST MS Search format. Unzip whole folder where the **NIST MS Search program** installed. If you do not have NIST MS Search Program see **//Notes//** below. |~1.44MB |[[https://chemdata.nist.gov/download/peptide_library/libraries/oligosaccharides/MS_Library_of_Oligosaccharides_in_human_milk_SRM_1953.zip|Download]] |
|2 |**Readme.pdf** - Short instruction for installation and use |~306KB |[[https://chemdata.nist.gov/download/peptide_library/libraries/oligosaccharides/Readme.pdf|Download]] |
|3 |md5_checklist.chk - MD5 hash file. |1KB |[[https://chemdata.nist.gov/download/peptide_library/libraries/oligosaccharides/md5_checklist.chk|Download]] |


** NOTES** 


You will need **NIST MS Search software v.2.3**  to search the oligosaccharides library. The software can be download from here: [[https://chemdata.nist.gov/download/peptide_library/libraries/glycopeptide/NIST_IgG_Glycopeptide.zip|NIST_IgG_Glycopeptide.zip]]. Download archive and unzip installation file. Run NISTmAb_Mass_Spectral_Library.exe to install the search program.


Release Date: July 20, 2018


<WRAP right lo>For questions, please contact  \\ Dr. Connie A. Remoroza   \\ (email: concepcion.remoroza@nist.gov) </WRAP>


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