===== Glycopeptide Mass Spectral Libraries =====

Libraries constructed from the analyses of intact glycopeptides derived from human IgG1 mAb drugs, human milk, SARS-CoV-2 spike, hemagglutinin and neuraminidase glycoproteins have been made available for download.  For experimental details, please see publication associated with each library.

Note, human milk, SARS-CoV-2 spike, hemagglutinin and neuraminidase glycopeptide libraries include glycopeptide abundance distribution spectra (GADS).  These libraries capture the distribution of glycoforms observed for a given glycosylation site and may be viewed and searched using NIST MS Search.  Further, they use retention time and MS1-level information to annotate potential false positive identifications. 

^Description  ^Associated Publication ^Library\\ Type(S) ^Release/\\ Details^ 
|Glycan\\ Distributions of\\ Glycopeptides|**Representing and comparing site-specific glycan abundance distributions of glycoproteins.**\\ //Remoroza, Concepcion A., Burke, Meghan C., Liu, Yi, Mirokhin, Yuri A., Tchekhovskoi, Dmitrii V., Yang, Xiaoyu, Stein, Stephen E.//\\ Mass Spectrometry Data Center, Biomolecular Measurement Division, National Institute of Standards and Technology, Gaithersburg, MD, 20899-8362, USA ([[https://pubs.acs.org/doi/full/10.1021/acs.jproteome.1c00442|DOI]]) |GADS |07-19-2021\\ [[peptidew:nistmsgads|Details>>]]|
|Human IgG1\\ mAb Drugs |**In-Depth Characterization and Spectral Library Building of Glycopeptides in the Tryptic Digest of a Monoclonal Antibody Using 1D and 2D LC-MS/MS.**\\ //Dong Q, Yan X, Liang Y, and Stein E. Stein//\\ J Proteome Res. 2016, 15 (5),1472-86; [[https://pubs.acs.org/doi/full/10.1021/acs.jproteome.5b01046|DOI]]. |MSMS  |Nov, 2015\\ [[peptidew:lib:newigghsalib|Details>>]]  |
|Human Milk\\ N-linked \\ Glycopeptides |**Mass Spectra Library-Based Methods for Analysis of Site-specific N-Glycosylation Applied to Human Milk Proteins.**\\ //Concepcion A. Remoroza, Meghan C. Burke, Xiaoyu Yang, Sergey Sheetlin, Yuri Mirokhin, Sanford P. Markey, Dmitrii V. Tchekhovskoi, Stephen E. Stein//\\ Mass Spectrometry Data Center, Biomolecular Measurement Division, National Institute of Standards and Technology, Gaithersburg, MD, 20899-8362, USA ([[https://pubs.acs.org/doi/full/10.1021/acs.jproteome.2c00286|DOI]]) |MSMS\\ GADS |  02-26-2022\\ [[peptidew:human_n_linked_glyco|Details>>]]  | 
|SARS-CoV-2\\ Spike Protein\\ Glycopeptides |**Determining Site-Specific Glycan Profiles of Recombinant SARS-CoV-2 Spike Proteins from Multiple Sources.**\\ //Meghan C. Burke, Yi Liu, Concepcion Remoroza, Yuri A. Mirokhin, Sergey L. Sheetlin, Dmitrii V. Tchekhovskoi, Guanghui Wang, Xiaoyu Yang, Stephen E. Stein.//\\ Mass Spectrometry Data Center, Biomolecular Measurement Division, National Institute of Standards and Technology, Gaithersburg, MD, 20899-8362, USA ([[https://pubs.acs.org/doi/10.1021/acs.jproteome.3c00271|DOI]]). |MSMS\\ GADS |  04-04-2023\\ [[peptidew:sars-cov-2_spike_protein_n_linked_glyco|Details>>]]  | 
|Hemagglutinin and \\ Neuraminidase \\ Glycopeptides   |**Variation of Site-Specific Glycosylation Profiles of Recombinant Influenza Glycoproteins.**\\ //Zachary C. Goecker, Meghan C. Burke, Concepcion A. Remoroza, Yi Liu, Yuri A. Mirokhin, Sergey L. Sheetlin, Dmitrii V. Tchekhovskoi, Xiaoyu Yang, Stephen E. Stein.//\\ Mass Spectrometry Data Center, Biomolecular Measurement Division, National Institute of Standards and Technology, Gaithersburg, MD, 20899-8362, USA (submitted for publication). |MSMS\\ GADS |  05-16-2023\\ [[peptidew:influenza_a_glycoproteins|Details>>]] |