====== Human Hair Peptide Spectral Library ======
===== Peptide Library (Sep. 17, 2019) =====
The human hair peptide spectral library was created from data generated at Mass Spectrometry Data Center at National Institute of Standards and Technology (NIST).


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^ File - Description ^ Size ^ Link ^
| **human_hair_selected_with_GVPs_passed_msp.tar.gz** \\ Consensus library in plain ASCII text (msp format). This format can be used as an exchange format. It is also directly readable by some software applications, including Skyline, after unzipping. |  19MB| [[https://chemdata.nist.gov/download/peptide_library/libraries/human/HCD/hair/human_hair_selected_with_GVPs_passed_msp.tar.gz|Download]] |
| **human_hair_selected_with_GVPs_passed.tar.gz** \\ Consensus library in NIST binary format. The folder generated by extracting this package can be used directly by [[peptidew:mspepsearch|MS Search or MSPepSearch]] and by the MSPepSearch node within Proteome Discoverer. |  46MB| [[https://chemdata.nist.gov/download/peptide_library/libraries/human/HCD/hair/human_hair_selected_with_GVPs_passed.tar.gz|Download]] | 
| **uniprot-all_Hair_Published_GVPs_fasta.tar.gz** \\ FASTA file used to search human hair samples.|  7MB|[[https://chemdata.nist.gov/download/peptide_library/libraries/human/HCD/hair/uniprot-all_Hair_Published_GVPs_fasta.tar.gz|Download]]|
| **md5_checklist.chk** - MD5 hash file. |  1KB| [[https://chemdata.nist.gov/download/peptide_library/libraries/human/HCD/hair/md5_checklist.chk|Download]] |
 
</WRAP>

  * Total number of Spectra: **6,280**
  * Unique Peptide Sequences: **2,240**
  * Total Proteins in FASTA file used: **20,183**
  * Proteome Sequence Coverage: **70%**

===== Decoy Library (Feb. 21, 2020) =====
The decoy library (sequence reversed) was created based on the methods described in the paper: \\ 
**Zhang, Zheng, et al.** //"Reverse and random decoy methods for false discovery rate estimation in high mass accuracy peptide spectral library searches."// Journal of Proteome Research 17.2 (2018): 846-857.

<WRAP center group 90%>
^ File - Description ^ Size ^ Link ^
| **human_hair_selected_with_GVPs_passed_decoy.msp.tar.gz** \\ Library of decoy spectra in plain ASCII text (msp format). This format can be used as an exchange format. It is also directly readable by some software applications, including Skyline, after unzipping. |  19MB| [[https://chemdata.nist.gov/download/peptide_library/libraries/human/HCD/decoy/Orbitrap_Fusion_Lumos-HCD_hair/human_hair_selected_with_GVPs_passed_decoy.msp.tar.gz|Download]] |
| **human_hair_selected_with_GVPs_passed_decoy.tar.gz** \\ Library of decoy spectra in NIST binary format. The folder generated by extracting this package can be used directly by [[peptidew:mspepsearch|MS Search or MSPepSearch]] and by the MSPepSearch node within Proteome Discoverer. |  46MB| [[https://chemdata.nist.gov/download/peptide_library/libraries/human/HCD/decoy/Orbitrap_Fusion_Lumos-HCD_hair/human_hair_selected_with_GVPs_passed_decoy.tar.gz|Download]] | 
| **md5_checklist.chk** - MD5 hash file. |  1KB| [[https://chemdata.nist.gov/download/peptide_library/libraries/human/HCD/decoy/Orbitrap_Fusion_Lumos-HCD_hair/md5_checklist.chk|Download]] |
 
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