====== The IARPA Proteos Human Skin and Hair Library ======
===== Peptide Consensus HCD Spectral Library (Oct. 01, 2021) =====
This consensus spectral library was derived from 629 raw data files generated on Orbitrap mass spectrometers, which include


  * 536 DDA and PRM raw data files for skin samples from the IARPA Proteos Program’s phases 1, 2, and 3. Data were generated by Signature Science and University of Washington using skin samples provided by Lawrence Livermore National Laboratory. [[https://www.iarpa.gov/index.php/research-programs/proteos|Click here for more information on the IARPA Proteos Program]].

  * 19 DDA raw data files from LCMS analyses of Proteos skin samples at the Mass Spectrometry Data Center, NIST 

  * 74 DDA raw data files from LCMS analyses of human hair samples at the Mass Spectrometry Data Center, NIST

The library contains **27,971** consensus spectra that correspond to **20,131** distinct peptide sequences including 239 GVPs. The library was divided into three sub-libraries based on spectral quality score, number of miscleavages in a peptide sequence, and whether a peptide is tryptic or semi-tryptic.


<WRAP center group 90%>
^ File - Description ^ Size ^ Link ^
| Library 1 (best) NIST Library format: \\ <color green>IARPA3_best_tissue_add_info.zip</color> \\ **8346** high-quality spectra, **6202** mostly \\ tryptic peptides without missed cleavages. |  25MB| [[https://chemdata.nist.gov/download/peptide_library/libraries/skin_hair/IARPA3_best_tissue_add_info.zip|Download]] |
| Library 1 (best) MSP text format: \\ <color green>IARPA3_best_tissue_add_info.msp.zip</color> \\ **8346** high-quality spectra, **6202** mostly \\ tryptic peptides without missed cleavages. |  9.2MB| [[https://chemdata.nist.gov/download/peptide_library/libraries/skin_hair/IARPA3_best_tissue_add_info.msp.zip|Download]] |
| Library 2 (good) NIST Library format: \\ <color green>IARPA3_good_tissue_add_info.zip</color> \\ **9348** medium-quality spectra, **7503** mostly \\ tryptic peptides with missed cleavages. |  29MB| [[https://chemdata.nist.gov/download/peptide_library/libraries/skin_hair/IARPA3_good_tissue_add_info.zip|Download]] |
| Library 2 (good) MSP text format: \\ <color green>IARPA3_good_tissue_add_info.msp.zip</color> \\ **9348** medium-quality spectra, **7503** mostly \\ tryptic peptides with missed cleavages. |  11MB| [[https://chemdata.nist.gov/download/peptide_library/libraries/skin_hair/IARPA3_good_tissue_add_info.msp.zip|Download]] |
| Library 3 (semi-tryptic) NIST Library format: \\ <color green>IARPA3_semitryptic_tissue_add_info.zip</color>  \\ **10277** high- and medium-quality spectra, \\ **7876** mostly semi-tryptic peptides. |  15MB| [[https://chemdata.nist.gov/download/peptide_library/libraries/skin_hair/IARPA3_semitryptic_tissue_add_info.zip|Download]] |
| Library 3 (semi-tryptic) MSP text format: \\ <color green>IARPA3_semitryptic_tissue_add_info.msp.zip</color>  \\ **10277** high- and medium-quality spectra, \\ **7876** mostly semi-tryptic peptides. |  15MB| [[https://chemdata.nist.gov/download/peptide_library/libraries/skin_hair/IARPA3_semitryptic_tissue_add_info.msp.zip|Download]] |
| <color green>SwissProt_human_20371_2021 ..... fasta.zip</color> \\ FASTA file used to create the library. |  7.3MB| [[https://chemdata.nist.gov/download/peptide_library/libraries/skin_hair/SwissProt_human_20371_2021August24_plus_combined_skin_hair_GVPs_2648.fasta.zip|Download]] |
| <color green>IARPA3_all.out.zip</color> \\ Text file listing the MS/MS scan numbers and associated raw \\ data file names for each consensus spectrum in the library. |  12MB| [[https://chemdata.nist.gov/download/peptide_library/libraries/skin_hair/IARPA3_all.out.zip|Download]] |
| <color green>Library_GVP_list.tsv.zip</color> \\ Text file listing GVPs in the library that came from IARPA Proteos panels. |  2.2KB| [[https://chemdata.nist.gov/download/peptide_library/libraries/skin_hair/Library_GVP_list.tsv.zip|Download]] |
| <color green>md5_checklist.chk</color> - MD5 hash file. |  1KB| [[https://chemdata.nist.gov/download/peptide_library/libraries/skin_hair/md5_checklist.chk|Download]] |
 
</WRAP>


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