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— peptidew:lib:kustersynselected20170530 [2021/07/08 00:41] (current)
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 +====== Human Synthetic Peptide Spectral Library ======
 +=====  Peptide Library (May 30, 2017) =====
 +
 +The human synthetic peptide spectral library was created from data deposited with the ProteomeXchange Consortium (http://proteomecentral.proteomexchange.org) via the PRIDE partner repository with the data set identifier PXD004732.
 +
 +Reference: D. Zolg … B. Kuster, Nature Methods, 14, 259 (2017): “Building ProteomeTools based on a complete synthetic human proteome”
 +
 +{{page>peptidew:lib:newfiles_warning}}
 +
 +<WRAP center group 80%>
 +^ File - Description ^ Size ^ Link ^
 +| **human_synthetic_hcd_selected.msp.tar.gz** \\ Library of selected spectra in plain ASCII text (msp format). This format can be used as an exchange format. It is also directly readable by some software applications, like Skyline, after unzipping. |  2.2GB| [[https://chemdata.nist.gov/download/peptide_library/libraries/human/HCD/2017_05_30/human_synthetic_hcd_selected.msp.tar.gz|Download]] |
 +| **human_synthetic_hcd_selected.tar.gz** \\ The Library of selected spectra in NIST binary format. The folder generated by extracting this package can be used directly by [[peptidew:mspepsearch|MS Search or MSPepSearch]] and by the MSPepSearch node within Proteome Discoverer. |  4.9GB| [[https://chemdata.nist.gov/download/peptide_library/libraries/human/HCD/2017_05_30/human_synthetic_hcd_selected.tar.gz|Download]] | 
 +| **uniprot-all.fasta.tar.gz** \\ FASTA file used to create the library.|  7.2MB| [[https://chemdata.nist.gov/download/peptide_library/libraries/human/HCD/2017_05_30/uniprot-all.fasta.tar.gz|Download]] | 
 +| **md5_checklist.chk** - MD5 hash file. |  1KB| [[https://chemdata.nist.gov/download/peptide_library/libraries/human/HCD/2017_05_30/md5_checklist.chk|Download]] | 
 +</WRAP>
 +
 +  * Total number of Spectra: **696,692**
 +  * Unique Peptide Sequences: **188,805**
 +  * Total Proteins in FASTA file used: **20,183**
 +  * Proteome Sequence Coverage: **20.63%**
 +
 +
 +=====  Decoy Library (Feb 21, 2020) =====
 +
 +The decoy library (sequence reversed) was created based on the methods described in the paper: \\ 
 +**Zhang, Zheng, et al.** //"Reverse and random decoy methods for false discovery rate estimation in high mass accuracy peptide spectral library searches."// Journal of Proteome Research 17.2 (2018): 846-857.
 +
 +<WRAP center group 80%>
 +^ File - Description ^ Size ^ Link ^
 +| **human_synthetic_hcd_selected_decoy.msp.tar.gz** \\ Library of decoy spectra in plain ASCII text (msp format). This format can be used as an exchange format. It is also directly readable by some software applications, like Skyline, after unzipping. |  2.2GB| [[https://chemdata.nist.gov/download/peptide_library/libraries/human/HCD/decoy/Orbitrap_Fusion_Lumos-HCD_synthetic/human_synthetic_hcd_selected_decoy.msp.tar.gz|Download]] |
 +| **human_synthetic_hcd_selected_decoy.tar.gz** \\ Library of decoy spectra in NIST binary format. The folder generated by extracting this package can be used directly by [[peptidew:mspepsearch|MS Search or MSPepSearch]] and by the MSPepSearch node within Proteome Discoverer. |  4.6GB| [[https://chemdata.nist.gov/download/peptide_library/libraries/human/HCD/decoy/Orbitrap_Fusion_Lumos-HCD_synthetic/human_synthetic_hcd_selected_decoy.tar.gz|Download]] | 
 +| **md5_checklist.chk** - MD5 hash file. |  1KB| [[https://chemdata.nist.gov/download/peptide_library/libraries/human/HCD/decoy/Orbitrap_Fusion_Lumos-HCD_synthetic/md5_checklist.chk|Download]] | 
 +</WRAP>
 +
 +
 +{{page>pepcopyright}}
 +
 +
  
peptidew/lib/kustersynselected20170530.txt · Last modified: 2021/07/08 00:41 (external edit)

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