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peptidew:lib:hsa_it [2018/01/30 16:38]
 peptidew:lib:hsa_it [2021/07/08 22:42] (current)
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 +====== Single Protein Library: The Human Serum Albumin  ======
 +=====  April 22, 2014 =====
 +
 +Over 3,000 CID tandem spectra for the human serum albumin (HAS) library was created from data generated at the NIST Mass Spectrometry Data Center. \\
 +
 +The library is available in two formats. Unzip after downloading. 
 +
 +//Reference: Mol. Cell. Proteomics 2014, 13 (9), 2435−49; doi: 10.1074/mcp.O113.037135. . Tandem mass spectral libraries of peptides in digests of individual proteins: Human Serum Albumin (HSA).  Dong Q, Yan X, Kilpatrick LE, Liang Y, Mirokhin YA, Roth JS, Rudnick PA, Stein SE
 +//
 +
 +
 +<WRAP center group 90%>
 +^ File - Description ^ Size ^ Link ^
 +| **NIST14_Peptide_HSA_Library.zip** - ZIP Archive for Installation of Windows Database Program and Library.  The folder generated by extracting this package can be used directly by [[peptidew:mspepsearch|MS Search or MSPepSearch]] and by the MSPepSearch node within Proteome Discoverer.|  ~19MB | [[https://chemdata.nist.gov/download/peptide_library/libraries/2014/NIST14_Peptide_HSA_Library.zip|Download]] | 
 +| **hsa20140422.zip** - ZIP Archive with Library. Consensus library in plain ASCII text (msp format). This format can be used as an exchange format. It is also directly readable by some software applications, including Skyline, after unzipping. (.MSP text format) . |  ~6MB | [[https://chemdata.nist.gov/download/peptide_library/libraries/2014/hsa20140422.zip|Download]] | 
 +| **md5_checklist.chk** - MD5 hash file. |  1KB | [[https://chemdata.nist.gov/download/peptide_library/libraries/2014/md5_checklist.chk|Download]] |
 +
 +</WRAP>
 +
 +  * Total number of Spectra: **3040**
 +  * Unique Peptides: **2073**
 +  * Sequence Coverage: **100%**
 +
 +----
 +
 +To install the Database Program and Library after downloading and unzipping, click on //NIST14_Peptide_HSA_Library.exe// and then follow instructions.
 +
 +MS1 Statistics for HSA Peptide Identifications is available  {{:peptidew:HSASingleProteinLibrary_MS1DataAnalysis.pdf|here}}.
 +
 +Additional documentation for NIST peptide libraries is {{:peptidew:nist_peplib_6_14.pdf|here}}.
 +
 +For more installation details {{:peptidew:readme_hsa.pdf|readme}}
 +
 +{{page>pepcopyright}}
  

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