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Human Synthetic Peptide Spectral Library
Peptide Library (May 30, 2017)
The human synthetic peptide spectral library was created from data deposited with the ProteomeXchange Consortium (http://proteomecentral.proteomexchange.org) via the PRIDE partner repository with the data set identifier PXD004732.
Reference: D. Zolg … B. Kuster, Nature Methods, 14, 259 (2017): “Building ProteomeTools based on a complete synthetic human proteome”
The libraries in the msp and NIST binary formats were updated on July 25, 2018. Previous versions of these files are available on request.
| File - Description | Size | Link |
|---|---|---|
| human_synthetic_hcd_selected.msp.tar.gz Library of selected spectra in plain ASCII text (msp format). This format can be used as an exchange format. It is also directly readable by some software applications, like Skyline, after unzipping. | 2.2GB | Download |
| human_synthetic_hcd_selected.tar.gz The Library of selected spectra in NIST binary format. The folder generated by extracting this package can be used directly by MS Search or MSPepSearch and by the MSPepSearch node within Proteome Discoverer. | 4.9GB | Download |
| uniprot-all.fasta.tar.gz FASTA file used to create the library. | 7.2MB | Download |
| md5_checklist.chk - MD5 hash file. | 1KB | Download |
- Total number of Spectra: 696,692
- Unique Peptide Sequences: 188,805
- Total Proteins in FASTA file used: 20,183
- Proteome Sequence Coverage: 20.63%
Decoy Library (Feb 21, 2020)
The decoy library (sequence reversed) was created based on the methods described in the paper:
Zhang, Zheng, et al. “Reverse and random decoy methods for false discovery rate estimation in high mass accuracy peptide spectral library searches.” Journal of Proteome Research 17.2 (2018): 846-857.
| File - Description | Size | Link |
|---|---|---|
| human_synthetic_hcd_selected_decoy.msp.tar.gz Library of decoy spectra in plain ASCII text (msp format). This format can be used as an exchange format. It is also directly readable by some software applications, like Skyline, after unzipping. | 2.2GB | Download |
| human_synthetic_hcd_selected_decoy.tar.gz Library of decoy spectra in NIST binary format. The folder generated by extracting this package can be used directly by MS Search or MSPepSearch and by the MSPepSearch node within Proteome Discoverer. | 4.6GB | Download |
| md5_checklist.chk - MD5 hash file. | 1KB | Download |
© Copyright information
These mass spectral libraries are protected by copyright law and may not be re-distributed without a valid Distribution Agreement. To receive such an agreement, contact the Standard Reference Data Program at the National Institute of Standards and Technology by emailing data@nist.gov or calling 301-975-2008.
