Human Synthetic Peptide Spectral Library

(May 30, 2017)

The human synthetic peptide spectral library was created from data deposited with the ProteomeXchange Consortium ( via the PRIDE partner repository with the data set identifier PXD004732.

Reference: D. Zolg … B. Kuster, Nature Methods, 14, 259 (2017): “Building ProteomeTools based on a complete synthetic human proteome”

The libraries in the msp and NIST binary formats were updated on July 25, 2018. Previous versions of these files are available on request.

File - Description Size Link
human_synthetic_hcd_selected.msp.tar.gz - Library of selected spectra in plain ASCII text (msp format). This format can be used as an exchange format. It is also directly readable by some software applications, like Skyline, after unzipping. 2.2GB Download
human_synthetic_hcd_selected.tar.gz - The Library of selected spectra in NIST binary format. The folder generated by extracting this package can be used directly by MS Search or MSPepSearch and by the MSPepSearch node within Proteome Discoverer. 4.9GB Download
uniprot-all.fasta.tar.gz - FASTA file used to create the library. 7.2MB Download
md5_checklist.chk - MD5 hash file. 1KB Download
  • Total number of Spectra: 696,692
  • Unique Peptide Sequences: 188,805
  • Total Proteins in FASTA file used: 20,183
  • Proteome Sequence Coverage: 20.63%

© Copyright information
These mass spectral libraries are protected by copyright law and may not be re-distributed without a valid Distribution Agreement. To receive such an agreement, contact the Standard Reference Data Program at the National Institute of Standards and Technology by emailing or calling 301-975-2008.

peptidew/lib/kustersynselected20170530.txt · Last modified: 2018/08/07 12:53 (external edit)

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