The NISTmAb Mass Spectral Library of Human IgG1κ mAb Drugs

A mass spectral library composed of all identifiable spectra derived from the tryptic digest of the NISTmAb IgG1κ were created from over six million peptide-spectrum matches acquired by high-resolution, accurate-mass 1D/2D LC-MS/MS analyses. The library contains a selection of 12,600 high quality HCD spectra of 3,300 peptide ions.

Reference: MAbs. 2018 Apr;10(3):354-369. The NISTmAb tryptic peptide spectral library for monoclonal antibody characterization. Dong Q, Liang Y, Yan X, Markey SP, Mirokhin YA, Tchekhovskoi DV, Bukhari TH, Stein SE.

File Size Link to download
1 - Archive with Library in NIST MS Search format. For the program itself see Notes* section below. ~48MB Download
2 - Archive for Installation of Windows Database Program and Library. Download archive and unzip installation file. Run NISTmAb_Mass_Spectral_Library.exe to install the search program and database ~19MB Download
3 md5_checklist.chk - MD5 hash file. 1KB Download


  1. NIST MS Search program with previous (2018) version of the library still available for download (65MB). Download archive and unzip installation file. Run executable for installation of the program and the library. Newer version of the library can be copied over the old one.
  2. Little bit more information about the program is in the **Readme.pdf** file.

© Copyright information
These mass spectral libraries are protected by copyright law and may not be re-distributed without a valid Distribution Agreement. To receive such an agreement, contact the Standard Reference Data Program at the National Institute of Standards and Technology by emailing or calling 301-975-2008.

peptidew/lib/human_igg1k_mab_drugs.txt · Last modified: 2021/07/08 23:06 (external edit)

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