Mass Spectrometry Data center
Over 1,700 HCD energy-dependent spectra for 81 different glycans attached to different tryptic peptide sequence motifs are contained in the glycopeptide library of human and recombinant IgG antibodies.
The library is available in a .ZIP archive as part of NIST MS Search, a full-featured Microsoft Windows® application.
Reference: J Proteome Res. 2016, 15 (5),1472-86; doi: 10.1021/acs.jproteome.5b01046. In-Depth Characterization and Spectral Library Building of Glycopeptides in the Tryptic Digest of a Monoclonal Antibody Using 1D and 2D LC-MS/MS. Dong Q, Yan X, Liang Y, and Stein SE.
|File||Size||Link to download|
|1||NIST_IgG_Glycopeptide.zip - Archive for Installation of Windows Database Program and Library. Download archive and unzip installation file. Run NIST_IgG_Glycopeptide.exe to install the search program and database||~12MB||Download|
|2||Readme.pdf - Short instruction for installation and use||~214KB||Download|
|3||md5_checklist.chk - MD5 hash file.||1KB||Download|
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These mass spectral libraries are protected by copyright law and may not be re-distributed without a valid Distribution Agreement. To receive such an agreement, contact the Standard Reference Data Program at the National Institute of Standards and Technology by emailing email@example.com or calling 301-975-2008.