MS_Piano (Mass Spectrum Peptide Annotation) is a comprehensive software for fast and reliable annotation of high- and low- resolution tandem mass spectra of CID and ETD in positive and negative modes for peptides, N- and O- linked glycopeptides. It annotates each peak in a spectrum with possible product ion(s) and the mass difference between the measured and theoretical m/z values. It also measures the spectral quality with various parameters. This software has been used as an essential software tool for building NIST Libraries of Peptides and Glycopeptides. MS_Piano is freely available for the Windows operating system.

MS_Piano Version 4.3 download: ms_piano_exe.zip

• Software tool convert2msp to convert the files from MS_Fragger and pGlyco searching respectively to the msp files for annotation is also included.
• NISTMS-GADS for spectral visualization and glycopeptide library (included) searching
• NIST Peptide Libraries

Previous Version:
MS_Piano Version 1.0 download: ms_piano_exe1.0.zip
MS_Piano_dll.zip (for programmers use): ms_piano_dll.zip

Reference:
MS_Piano: A Software Tool for Annotating Peaks in CID Tandem Mass Spectra of Peptides and N-Glycopeptides.
Xiaoyu Yang, Pedatsur Neta, Yuri A. Mirokhin, Dmitrii V. Tchekhovskoi, Concepcion A. Remoroza, Meghan C. Burke, Yuxue Liang, Sanford P. Markey, and Stephen E. Stein
Journal of Proteome Research 2021 20 (9), 4603-4609. DOI: 10.1021/acs.jproteome.1c00324

For questions, please contact
Xiaoyu (Sara) Yang
(email: xiaoyu.yang@nist.gov)