peptidew:clib:humanhcditraqphosphoselected20141126

Human HCD iTRAQ Phospho Library

Peptide Library (Nov. 26, 2014)

The human HCD iTRAQ Phospho library was created entirely from raw data stored on the Clinical Proteomic Tumor Analysis Consortium (CPTAC) data portal https://cptac-data-portal.georgetown.edu/cptacPublic/. Contributing laboratories were the Broad Institute and Pacific Northwest National Laboratory.

The libraries in the msp and NIST binary formats were updated on July 25, 2018. Previous versions of these files are available on request.

File - Description Size Link
cptac2_human_hcd_itraq_phospho_selected.msp.tar.gz - Consensus library in plain ASCII text (msp format). This format can be used as an exchange format. It is also directly readable by some software applications, including Skyline, after unzipping. 1.1GB Download
cptac2_human_hcd_itraq_phospho_selected.tar.gz - Consensus library in NIST binary format. The folder generated by extracting this package can be used directly by MS Search or MSPepSearch and by the MSPepSearch node within Proteome Discoverer. 1.8GB Download
20111201-RefSeq-Human-37-Mouse-37-Trypsin.fasta.tar.gz - FASTA file used to search CompRef human-mouse xenograft samples.16.2MBDownload
md5_checklist.chk - MD5 hash file. 1KB Download
  • Total number of Spectra: 223,340
  • Unique Peptide Sequences: 67,533
  • Total Proteins in FASTA file used: 32,800

Decoy Library (Feb. 21, 2020)

The decoy library (sequence reversed) was created based on the methods described in the paper:
Zhang, Zheng, et al. “Reverse and random decoy methods for false discovery rate estimation in high mass accuracy peptide spectral library searches.” Journal of Proteome Research 17.2 (2018): 846-857.

File - Description Size Link
cptac2_human_hcd_itraq_phospho_selected_decoy.msp.tar.gz
Library of decoy spectra in plain ASCII text (msp format). This format can be used as an exchange format. It is also directly readable by some software applications, including Skyline, after unzipping.
1.1GB Download
cptac2_human_hcd_itraq_phospho_selected_decoy.tar.gz
Library of decoy spectra in NIST binary format. The folder generated by extracting this package can be used directly by MS Search or MSPepSearch and by the MSPepSearch node within Proteome Discoverer.
2.1GB Download
md5_checklist.chk - MD5 hash file. 1KB Download
peptidew/clib/humanhcditraqphosphoselected20141126.txt · Last modified: 2021/07/08 00:36 (external edit)

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