Human Phosphopeptide Label Free Library

( Feb. 14, 2019)

The human phosphopeptide spectral library was created from data deposited with the ProteomeXchange Consortium ( via the PRIDE partner repository with the data set identifier PXD000612.

Reference: Sharma K, D'Souza RC, Tyanova S, Schaab C, Wiśniewski JR, Cox J, Mann M. Ultradeep Human Phosphoproteome Reveals a Distinct Regulatory Nature of Tyr and Ser/Thr-Based Signaling. Cell Rep. 2014 Aug 20. pii: S2211-1247(14)00620-2 PubMed: 25159151

File - Description Size Link
human_hcd_labelfree_phospho_selected_passed.msp.tar.gz - Consensus library in plain ASCII text (msp format). This format can be used as an exchange format. It is also directly readable by some software applications, including Skyline, after unzipping. 240MB Download
human_hcd_labelfree_phospho_selected_passed.tar.gz - Consensus library in NIST binary format. The folder generated by extracting this package can be used directly by MS Search or MSPepSearch and by the MSPepSearch node within Proteome Discoverer. 540MB Download
uniprot-all.fasta.tar.gz - FASTA file used to search CompRef human-mouse xenograft samples. 7MBDownload
md5_checklist.chk - MD5 hash file. 1KB Download
  • Total number of Spectra: 66,922
  • Unique Peptide Sequences: 27,400
  • Total Proteins in FASTA file used: 20,183
  • Proteome Sequence Coverage: 3.9%

© Copyright information
These mass spectral libraries are protected by copyright law and may not be re-distributed without a valid Distribution Agreement. To receive such an agreement, contact the Standard Reference Data Program at the National Institute of Standards and Technology by emailing or calling 301-975-2008.

peptidew/lib/phoshopept_labelfree_20190214.txt · Last modified: 2019/02/15 09:54 (external edit)

Page Tools